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National Repository of Grey Literature

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Fully Automated Method for the Design of Ancestral Proteins Štěpánek, Martin ; Martínek, Tomáš (referee) ; Musil, Miloš (advisor) Stabilita proteinů je zásadní vlastností ovlivňující možnosti průmyslového použití proteinů. Tato práce se zaměřuje na vylepšení automatizovaného nástroje FireProtASR, který využívá rekonstrukci ancestrálních sekvencí pro návrh proteinů se zvýšenou stabilitou. Do tohoto nástroje byla přidána nově navržená metoda rekonstrukce successorů. Successory představují sekvence proteinů, ke kterým bude v budoucnosti směřovat evoluce. Podle současných poznatků by měly mít vyšší aktivitu než současné proteiny, společně s vyšší schopností vázat se specificky na určité molekuly. Dále bylo vytvořeno nové webové uživatelské rozhraní, které poskytuje rychlé a intuitivní ovládání nástroje FireProtASR. Detailed record

Fully Automated Method for the Design of Ancestral Proteins Štěpánek, Martin ; Martínek, Tomáš (referee) ; Musil, Miloš (advisor) Stabilita proteinů je zásadní vlastností ovlivňující možnosti průmyslového použití proteinů. Tato práce se zaměřuje na vylepšení automatizovaného nástroje FireProtASR, který využívá rekonstrukci ancestrálních sekvencí pro návrh proteinů se zvýšenou stabilitou. Do tohoto nástroje byla přidána nově navržená metoda rekonstrukce successorů. Successory představují sekvence proteinů, ke kterým bude v budoucnosti směřovat evoluce. Podle současných poznatků by měly mít vyšší aktivitu než současné proteiny, společně s vyšší schopností vázat se specificky na určité molekuly. Dále bylo vytvořeno nové webové uživatelské rozhraní, které poskytuje rychlé a intuitivní ovládání nástroje FireProtASR. Detailed record

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